Publications

 

Schwartz, A. B., Kapur, A., Huang, Z., Anangi, R., Spear, J. M., Stagg, S., Fadone, E., Dekan, Z., Rosenberg, J. T., Grant, S. C., King, G. F., Mattousi, H., & Fadool, D. A. (2020). Olfactory bulb-targeted quantum dot (QD) bioconjugate and Kv1.3 blocking peptide improve metabolic health in obese male mice. J Neurochem.

 

Gao, Y., Guo, C., Watzlawik, J. O., Randolph, P. S., Lee, E. J., Huang, D., Stagg, S. M., Zhou, Huan-Xiang, Rosenberry, T., & Paravastu, A. K. (2020). Out-of-Register Parallel β-Sheets and Antiparallel β-Sheets Coexist in 150-kDa Oligomers Formed by Amyloid-β(1-42). J Mol Biol.

 

Paraan, M., Mendez, J., Sharum, S., Kurtin, D., He, H., & Stagg, S. M. (2020). The structures of natively assembled clathrin-coated vesicles. Science Advances. Retrieved from https://doi.org/10.1126/sciadv.aba8397 doi:10.1126/sciadv.aba8397

 

Zhang, Y., Sui, X., Stagg, S. M., & Zhang, J. (2020). FTIP: an accurate and efficient method for global protein surface comparison. Bioinformatics.

 

Randolph, P. S., & Stagg, S. M. (2020). Reconstruction of Average Subtracted Tubular Regions (RASTR) enables structure determination of tubular filaments by cryoEM. Journal of Structural Biology: X, 4, 100023. Retrieved from https://doi.org/10.1016%2Fj.yjsbx.2020.100023 doi:10.1016/j.yjsbx.2020.100023

 

Yu, G., Zhao, Y., Tian, S., Rai, J., He, H., Spear, J., Sousa, D., Fan, J., Yu, Hong-Guo, Stagg, S. M., & Li, H. (2019). Yeast R2TP Interacts with Extended Termini of Client Protein Nop58p. Sci Rep.

 

Nosrati, M., Dey, D., Mehrani, A., Strassler, S. E., Zelinskaya, N., Hoffer, E. D., Stagg, S. M., Dunham, C. M., & Conn, G. L. (2019). Functionally critical residues in the aminoglycoside resistance-associated methyltransferase RmtC play distinct roles in 30S substrate recognition. Journal of Biological Chemistry, 294(46), 17642-17653. Retrieved from https://doi.org/10.1074%2Fjbc.ra119.011181 doi:10.1074/jbc.ra119.011181

 

Mendez, J. H., Mehrani, A., Randolph, P., & Stagg, S. (2019). Throughput and Resolution with a Next Generation Direct Electron Detector. International Union of Crystallography Journal. Retrieved from https://doi.org/10.1101%2F620617 doi:10.1101/620617

Meyer, N. L., Hu, G., Davulcu, O., Xie, Q., Noble, A. J., Yoshioka, C., Gingerich, D. S., Trzynka, A., David, L., Stagg, S. M., & Chapman, M. S. (2019). Structure of the gene therapy vector, adeno-associated virus with its cell receptor. eLife, 8, 1660.

 

Heymann, J. B., Marabini, R., Kazemi, M., Sorzano, C. O. S., Holmdahl, M., Mendez, J. H., Stagg, S. M., Jonic, S., Palovcak, E., Armache, J.-P., Zhao, J., Cheng, Y., Pintilie, G., Chiu, W., Patwardhan, A., & Carazo, J.-M. (2018). The first single particle analysis Map Challenge: A summary of the assessments. J Struct Biol, 204, 291–300.

 

Mendez, J. H., & Stagg, S. M. (2018). Assessing the quality of single particle reconstructions by atomic model building. J Struct Biol, 204, 276–282.

 

Paraan, M., Bhattacharya, N., Uversky, V. N., & Stagg, S. M. (2018). Flexibility of the Sec13/31 cage is influenced by the Sec31 C-terminal disordered domain. J Struct Biol, 204, 250–260.

 

Randolph, P., Mendez, J., & Stagg, S. M. (2018). The Past, Present, and Future of Sample Preparation for CryoEM. Reference Module in Life Sciences.

 

Slosarek, E. L., Schuh, A. L., Pustova, I., Johnson, A., Frankel, E. B., Bhattacharya, N., Hanna, M. G., Burke, J. E., Ruhl, D. A., Quinney, K., Block, S., Peotter, J. L., Chapman, E. R., Sheets, M. D., Butcher, S. E., Stagg, S. M., & Audhya, A. (2018). Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation. Cell Rep, 24, 2248–2260.

 

Stagg, S. M., & Mendez, J. H. (2018). Processing apoferritin with the Appion pipeline. J Struct Biol, 204, 85–89.

 

Alewijnse, B., et al. (2017). Best practices for managing large CryoEM facilities. J Struct Biol, 225-236.

 

Kizziah, J. L., Manning, K. A., Dearborn, A. D., Wall, E. A., Klenow, L., Hill, R. L. L., Spilman, M. S., Stagg, S. M., Christie, G. E., & Dokland, T. (2017). Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids. Viruses, 384. doi:10.3390/v9120384

 

Tian, S., Yu, G., He, H., Zhao, Y., Liu, P., Marshall, A. G., Demeler, B., Stagg, S. M., & Li, H. (2017). Pih1p-Tah1p Puts a Lid on Hexameric AAA+ ATPases Rvb1/2p. Structure, 1519-1529.

 

Xie, Q., Spear, J. M., Noble, A. J., Sousa, D. M., Meyer, N. L., Davulcu, O., Zhang, F., Linhardt, R. J., Chapman, M. S., & Stagg, S. M. (2017). The 2.8 Å Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparinoid Pentasaccharide. Molecular Therapy. Methods & Clinical Development, 1-12.

 

Lowry, T. W., Hariri, H., Prommapan, P., Kusi-Appiah, A., Vafai, N., Bienkiewicz, E. A., Van Winkle, D. H., Stagg, S. M., & Lenhert, S. (2016). Quantification of Protein-Induced Membrane Remodeling Kinetics In Vitro with Lipid Multilayer Gratings. Small, 1-10.

 

Mukhitov, N., Spear, J. M., Stagg, S. M., & Roper, M. G. (2016). Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy. Anal Chem, 88, 629-34.

 

Gianfelice, A., Le, P. H. B., Rigano, L. A., Saila, S., Dowd, G. C., McDivitt, T., Bhattacharya, N., Hong, W., Stagg, S. M., & Ireton, K. (2015). Host endoplasmic reticulum COPII proteins control cell-to-cell spread of the bacterial pathogen Listeria monocytogenes. Research Cellular Microbiology, 1-17.

 

Johnson, A., Bhattacharya, N., Hanna, M., Schuh, A. L., Pennington, J. G., Otegui, M. S., Stagg, S. M., & Audhya, A. (2015). TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. EMBO J, 811-27.

 

Noble, A. J., & Stagg, S. M. (2015). Automated batch fiducial-less tilt-series alignment in Appion using Protomo. Journal of Structural Biology, 192, 270-8.

 

Spear, J. M., Noble, A. J., Xie, Q., Sousa, D. R., Chapman, M. S., & Stagg, S. M. (2015). The influence of frame alignment with dose compensation on the quality of single particle reconstructions. Journal of Structural Biology, 192, 196-203.

 

Spear, J. M., Al Koborssy, D., Schwartz, A. B., Johnson, A. J., Audhya, A., Fadool, D. A., & Stagg, S. M. (2015). Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a. BMC Biochemistry, 16, 1-16.

 

Ramia, N. F., Spilman, M., Tang, L., Shao, Y., Elmore, J., Hale, C., Cocozaki, A., Bhattacharya, N., Terns, R. M., & Stagg, S. M. (2014). Essential Structural and Functional Roles of the Cmr4 Subunit in RNA Cleavage by the Cmr CRISPR- Cas Complex. Cell Reports, 9, 1-8.

 

Stagg, S. M., Noble, A. J., Spilman, M., & Chapman, M. S. (2014). ResLog plots as an empirical metric of the quality of cryo-EM reconstructions. Journal of Structural Biology, 418-426.

 

Hariri, H., Bhattacharya, N., Johnson, K., Noble, A. J., & Stagg, S. M. (2014). Insights into the Mechanisms of Membrane Curvature and Vesicle Scission by the Small GTPase Sar1 in the Early Secretory Pathway. Journal of Molecular Biology, 426, 3811–3826.

 

Noble, A. J., Zhang, Q., O'Donnell, J., Hariri, H., Bhattacharya, N., Marshall, A. G., & Stagg, S. M. (2013). A Pseudoatomic Model of the COPII Cage Obtained From Cryo-electron Microscopy and Mass Spectrometry. Nat Struct Mol Biol, 20, 167-173.

 

Sousa, D. R., Stagg, S. M., & Stroupe, M. E. (2013). Cryo-EM Structures of the Actin:Tropomyosin Filament Reveal the Mechanism for the Transition From C- to M-State. Journal of Molecular Biology, 4225, 4544-55.

 

Spilman, M., Cocozaki, A., Hale, C., Shao, Y., Ramia, N., Terns, R., Terns, M., Li, H., & Stagg, S. M. (2013). Structure of an RNA Silencing Complex of the CRISPR-Cas Immune System. Molecular Cell, 146-152.

 

Xie, Q., Spilman, M., Meyer, N. L., Lerch, T. F., Stagg, S. M., & Chapman, M. S. (2013). Electron Microscopy Analysis of a Disaccharide Analog complex Reveals Receptor Interactions of Adeno-Associated Virus. Journal of Structural Biology, 129-135.

 

Bhattacharya, N., O Donnell, J., & Stagg, S. M. (2012). The Structure of the Sec13/31 COPII Cage Bound to Sec23. J Mol Biol, 420, 324-334.

 

Clare, D. K., Vasishtan, D., Stagg, S. M., Quispe, J., Farr, G. W., Topf, M., Horwich, A. L., & Saibil, H. R. (2012). ATP-Triggered Conformational Changes Delineate Substrate-Binding and -Folding Mechanics of the GroEL Chaperonin. Cell, 149, 113-123.

 

Lerch, T., O'Donnell, J., Meyer, N., Xie, Q., Taylor, K., Stagg, S. M., & Chapman, M. (2012). Structure of AAV-DJ, a Retargeted Gene Therapy Vector: Cryo-Electron Microscopy at 4.5 Å Resolution. Structure, 20, 1310 - 1320.

 

McCraw, D. M., O'Donnell, J. K., Stagg, S. M., Taylor, K. A., & Chapman, M. S. (2012). Structure of adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20. Virology, 431, 40-49.

 

Shrum, D. C., Woodruff, B. W., & Stagg, S. M. (2012). Creating an infrastructure for high-throughput high-resolution cryogenic electron microscopy. Journal of Structural Biology, 180, 254-258.

 

O'Donnell, J., Maddox, K., & Stagg, S. M. (2011). The structure of a COPII tubule. J Struct Biol, 173(2), 358-64.

 

Stagg, S. M., Knappenberger, K., Dowgiallo, A., & Chandra, M. (2011). Three-Dimensional Interfacial Structure Determination of Hollow Gold Nanosphere Aggregates. J Phys Chem Lett 2, 23.

 

Lander, G. C., Stagg, S. M., Pulokas, C., Yoshioka, C., Irving, A., Voss, N. R., Cheng, A., Fellmann, D., Mulder, J., et al. (2009). Appion: an integrated, database-driven pipeline to facilitate EM image processing. J Struct Biol, 166, 95-102.

 

Clare, D. K., Stagg, S. M., Quispe, J., Farr, G. W., Horwich, A. L., & Saibil, H. R. (2008). Multiple States of a Nucleotide-Bound Group 2 Chaperonin. Structure, 16, 528-534.

 

Stagg, S. M., Lander, G. C., Quispe, J., Voss, N., Cheng, A., Bradlow, H., Bradlow, S., Carragher, B., & Potter, C. S. (2008). A test-bed for optimizing high-resolution single particle reconstructions. J Struct Biol, 163, 29-39.

 

Stagg, S. M., LaPointe, P., Razvi, A., Gürkan, C., Potter, C. S., Carragher, B., & Balch, W. E. (2008). Structural Basis for Cargo Regulation of COPII Coat Assembly. Cell, 134, 474-484.

 

Stagg, S. M., Gurkan, C., Fowler, D. M., LaPointe, P., Foss, T. R., Potter, C. S., Carragher, B., & Balch, W. E. (2006). Structure of the Sec13/31 COPII coat cage. Nature, 439, 234-8.

 

Stagg, S. M., Lander, G. C., Pulokas, J., Fellmann, D., Cheng, A., Quispe, J. D., Mallick, S. P., Avila, R. M., Carragher, B., & Potter, C. S. (2006). Automated cryoEM data acquisition and analysis of 284,742 particles of GroEL. J Struct Biol, 155, 470-81.